Hector F. DeLuca, professor of biochemistry at the University of Wisconsin in Madison, examines the fascinating history of the discovery and applications of Vitamin D. DeLuca also discusses the work conducted at his laboratory, which has been devoted to the understanding of metabolism and mechanism of action of vitamins A and D. Also touched upon is the creation of the Wisconsin Alumni Research Foundation, whose mission is to transform university research into real products that benefit the society at large.
Vitamin D: The New Old Natural Wonder Drug
Cellular Metabolism, Enzimes
Cellular Metabolism, Enzimes Enzymes allow many chemical reactions to occur within the homeostasis constraints of a living system. Enzymes function as organic catalysts. A catalyst is a chemical involved in, but not changed by, a chemical reaction. Many enzymes function by lowering the activation energy of reactions. By bringing the reactants closer together, chemical bonds may be weakened and reactions will proceed faster than without the catalyst.
Interview with Dr. Steven Clarke – Part 1 of 5
On January 31st, Cutting Edge Talk Show conducted an interview with Dr. Steven Clarke, a world authority on age-related protein damage and repair. Dr. Clarke’s major interest is understanding the biochemistry of the aging process. He is particularly interested in the generation of age-damaged proteins by spontaneous chemical reactions and the physiological role of cellular enzymes that can reverse at least some portion of the damage. For over thirty years his laboratory focused its efforts on the degradation of aspartic acid and asparagine residues and the subsequent metabolism of their racemized and isomerized derivatives. His laboratory is presently determining the biological role of protein methyltransferases that can initiate the conversion of D-aspartyl residues to the L-configuration as well as the conversion of isopeptide linkages to normal peptide bonds. Such “repair” reactions may greatly increase the useful lifetime of cellular proteins and may help insure organismal survival. Dr. Clarke presented at GTCbio’s New Applications for Aging Research conference in January 2008. His presentation received highest ratings and ignited active discussion. The Cutting Edge Talk Show was moved by Dr. Clarke’s outstanding speech and decided to schedule a separate interview. The interviewers briefly touch on Dr. Clarke’s work and ask questions that may be of interest to non-scientists.
Soil microbial metabolism and nutrient status in a Mediterranean area as affected by plant cover
Product Description
This digital document is a journal article from Soil Biology and Biochemistry, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
The Mediterranean area of Southern Italy is characterized by different natural plant covers that mainly reflect different successional stages (i.e. low maquis, high maquis, Quercus ilex wood) and managed areas with introduced plant species (such as Pinus species). Soil properties could be affected by plant cover types as well as by plant species. Our objective was to determine the relationships of plant cover types and plant species with the chemical and biological characteristics of the soil. In four neighbouring areas with different plant cover types (low maquis, pure high maquis, high maquis with pines and pinewood, with pines planted by foresters in both cases), soil samples were collected under different plant species in order to evaluate the effect of plant cover types and plant species on soil properties. Soil samples were analyzed for nutrient content, microbial biomass, soil potential respiration and enzymatic activity (phosphatase, arylsulphatase, @b-glucosidase and hydrolase activities) as well as for pH, water holding capacity (WHC) and cation exchange capacity (CEC). Application of cluster analysis and principal component analysis to the data revealed that the plant cover type was the key factor influencing soil properties more than plant species. In fact, the largest differences were observed between pure high maquis soils and all other soils, with pure high maquis soils generally showing the highest values of WHC, CEC, nutrient content, organic and microbial C, soil respiration, phosphatase, arylsulphatase and @b-glucosidase activities. The significantly lower values of these variables in the low maquis relative to the pure high maquis probably reflect the effect of ecological succession on soil. The high maquis with pine, differing from the pure high maquis only for the presence or absence of pine, showed values of soil physical, chemical and biological characteristics similar to those found in the low maquis, thus suggesting that the presence of pine retards soil development.
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Interactive effects of naphthalene treatment and the onset of vitellogenesis on energy metabolism in liver and gonad, and plasma steroid hormones of rainbow … Biochemistry and Physiology, Part C]
Product Description
This digital document is a journal article from Comparative Biochemistry and Physiology, Part C, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
The purpose of the study was to assess in female fish the possible interaction between treatment with a polycyclic aromatic hydrocarbon (PAH) like naphthalene and the onset of vitellogenesis. In a first experiment, female rainbow trout (Oncorhynchus mykiss) at stages 2-3 (previtellogenesis) or 4 (early vitellogenesis) were intraperitoneally injected (2 @ml g^-^1) with vegetable oil alone (control) or containing naphthalene (50 mg kg^-^1) to be sampled 3 h later. A second experiment was similarly designed but using fish intraperitoneally implanted (10 @ml g^-^1) with slow-release coconut oil implants alone (control) or containing 50 mg naphthalene kg^-^1 body mass that were sampled 3 days after injection. On each sampling time, plasma levels of cortisol and 17@b-estradiol, and several metabolic parameters in plasma, liver and gonad were assessed. In controls, early vitellogenic fish compared with previtellogenic fish displayed changes that in some cases are confirmatory of previous studies whereas in other cases provide new information in plasma (increased amino acid levels), liver (decreased capacity for exporting glucose and reduced amino acid levels) and gonad (decreased amino acid levels). Naphthalene treatment produced in previtellogenic fish decreased 17@b-estradiol levels in plasma, increased plasma glucose or decreased liver gluconeogenic capacity whereas no major effects were noticed on parameters involved in lipid, amino acid and lactate metabolism. Differential effects of naphthalene treatment were noticed in early vitellogenic fish such as decreased 17@b-estradiol and glucose levels in plasma, increased hexokinase and glucokinase and lack of changes in fructose 1,6-bisphosphatase activities in liver, and a lower decrease of amino acid levels in gonad. Those alterations produced by naphthalene treatment resulted in a decreased capacity for covering the energy demand of vitellogenesis in liver and gonad that could contribute to a delay and/or impairment of the onset of maturation.

